Composite

Part:BBa_K1867024:Design

Designed by: Shien Yang Lee   Group: iGEM15_NYU-AD   (2015-09-16)


pro_TnaA_TnaB_term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to sequence optimize to remove Biobrick restriction sites from sequence.


Source

Coding sequences from Ecocyc database, accessed at: http://biocyc.org/ECOLI/sequence?object=EG11005 and http://biocyc.org/ECOLI/sequence?object=EG11006


References